Efficient Algorithm for Primer Design
نویسندگان
چکیده
منابع مشابه
Efficient primer design algorithms
MOTIVATION Primer design involves various parameters such as string-based alignment scores, melting temperature, primer length and GC content. This entails a design approach from multicriteria decision making. Values of some of the criteria are easy to compute while others require intense calculations. RESULTS The reference point method was found to be tractable for trading-off between deviat...
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MOTIVATION Before performing a polymerase chain reaction experiment, a pair of primers to clip the target DNA subsequence is required. However, this is a tedious task as too many constraints need to be satisfied. Various kinds of approaches for designing a primer have been proposed in the last few decades, but most of them do not have restriction sites on the designed primers and do not satisfy...
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SUMMARY We have developed U-PRIMER, a primer design program, to compute a minimal primer set (MPS) for any given set of DNA sequences. The U-PRIMER algorithm, which uses automatic variable fixing and automatic redundant constraint elimination to tackle the binary integer programming problem associated with the MPS selection problem. The program has been tested successfully with 32 adipocyte dev...
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In this paper, a double orthogonal array based genetic algorithm is proposed to decrease the searching space and increase the feasible quality of primers. The key point of the proposed algorithm is to achieve the elitism goal by applying the orthogonal arrays (OAs) that is used in quality engineering with a small amount of experimental features. The result shows that the proposed algorithm conv...
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ژورنال
عنوان ژورنال: Southeast Europe Journal of Soft Computing
سال: 2013
ISSN: 2233-1859
DOI: 10.21533/scjournal.v2i2.33